| Course Type | Elective Course |
| Course Level | Master's Degree With Thesis |
| Year of Study | - |
| Semester | - |
| ECTS | 10 |
| Name of Lecturer(s) |
|
| Learning Outcomes |
At the end of this course, the students;
- have knowledge about plant tissue culture basic laboratory techniques
- learn media and cultural conditions in plant tissue culture
- gain knowledge about protoplast culture,haploid plant production, meristem culture,embryo culture, micropropagation
- have knowledge about seconder metabolites and germplast conservation
|
| Mode of Delivery | Face to face |
| Prerequisites | No |
| Recommended Optional Programme Components | none |
| Course Description | Throughout this course, basic knowledge and techniques about plant cell, tissue and organ culture, plant breeding, applications of plant tissue culture in biotechnology and plant development will be discussed and concepts such as nutritional media, sterilization techniques, explants, micropropagation, callus culture, organogenesis, embryogenesis, somatic variation, haploids, hybrids, protoplast fusion will be covered. |
| Course Contents |
| WEEK |
TOPICS |
|
1st Week |
1 Introduction to Plant Tissue Culture |
|
2nd Week |
2 Tissue Culture Basic Laboratory Techniques I |
|
3rd Week |
3 Tissue Culture Basic Laboratory Techniques II |
|
4th Week |
4 Media and Cultural Conditions in Plant Tissue Culture |
|
5th Week |
5 Applications of Plant Tissue Culture |
|
6th Week |
6 Organogenesis |
|
7th Week |
7 Somatic Embryogenesis |
|
8th Week |
8 Midterm |
|
9th Week |
9 Diffrences Between Organogenesis and Somatik Embryogenesis |
|
10th Week |
10 Micropropagation I |
|
11th Week |
11 Micropropagation II |
|
12th Week |
12 Protoplast Culture, Haploid Plant Production, Meristem Culture |
|
13th Week |
13 Embryo Culture, Seconder Metabolites,Germplasm Conservation I |
|
14th Week |
14 Embryo Culture, Seconder Metabolites,Germplasm Conservation II |
|
15th Week |
|
|
| Recommended or Required Reading | Plant Cell Culture: a Practical Approach, 2nd edn. Dixon, R.A. and Gonzalez, R.A. (Eds) (1995). IRL Press at Oxford University Press, Oxford, UK.
Plant Cell Culture. Collin, H.A.,Edwards, S. (1998). BIOS Scientific Publishers, Oxford, UK.
|
| Planned Learning Activities and Teaching Methods |
|
| Assessment Methods and Criteria |
|
Quantity |
Percentage (%) |
| Mid-term |
1 |
40 |
| Total(%) |
|
40 |
| Contribution of In-term Studies to Overall Grade(%) |
|
40 |
| Contribution of Final Examination to Overall Grade(%) |
|
60 |
| Total(%) |
|
100 |
|
| ECTS Allocated Based on Student Workload |
| Activities |
Quantity |
Duration (Hour) |
Total Workload (Hour) |
| Midterm exam |
1 |
2 |
2 |
| Preparation for Quiz |
1 |
10 |
10 |
| Individual or group work |
|
|
|
| Preparation for Final exam |
1 |
85 |
85 |
| Course hours |
14 |
3 |
42 |
| Preparation for Midterm exam |
1 |
66 |
66 |
| Laboratory (including preparation) |
|
|
|
| Final exam |
1 |
3 |
3 |
| Homework |
2 |
46 |
92 |
| Total Work Load | 300 |
| Total Work Load / 30 (h) | 10 |
| ECTS Credit of the Course | 10 |
|
| Language of Instruction | Turkish |
| Work Placement(s) | No |