Information Package
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Program of Molecular Biology and Genetics (English)

MBGE303

RESEARCH TECHNIQUES FOR MOLECULAR BIOLOGY AND GENETICS

Course Code
MBGE303
Semester
Fifth Term (Fall)
Theory+Practice
1 + 4
ECTS
8
Course TypeCompulsory Course
Course LevelBachelor's Degree
Year of Study3
SemesterFifth Term (Fall)
ECTS8
Name of Lecturer(s)
Learning Outcomes

At the end of this course, the students;

  1. Will be able to apply the steps of molecular cloning.
  2. Know how to detect gene transferred by molecular cloning, determine expression level and protein expression.
  3. Know how to do experimental studies with the obtained recombinant organism.
  4. Know expression analysis with real-time quantitative polymerase chain reaction (PCR) (qRT-PCR) and interprets the results.
Mode of DeliveryFace to face
PrerequisitesNo
Recommended Optional Programme ComponentsNo
Course DescriptionIn this course, it is aimed to learn the basic principles of molecular cloning, the screening of clones, and the basic experimental protocols for target gene expression analysis and to gain practical knowledge about these techniques.
Course Contents
WEEK TOPICS
1st Week Course description and primer design for target gene amplification
2nd Week Competent cell preparation
3rd Week Amplification of the target gene (PCR)
4th Week Plasmid isolation (vector extraction)
5th Week Digesting genes and plasmids
6th Week Ligation
7th Week Transformation
8th Week Screening of clones (colony PCR)
9th Week Verification of selected colonies
10th Week Functional analysis of the cloned gene, recombinant protein production
11th Week qRT-PCR
12th Week Homework presentation
13th Week Homework presentation
14th Week Homework presentation
Recommended or Required ReadingGene Cloning and DNA Analysis: An Introduction. T.A. Brown. Sixth/Seventh Edition.
Molecular Biotechnology, Principles and Applications of Recombinant DNA. Bernard R. Glick, Jack J. Pasternak, ASM Press, Washington, D.C., 2009.
Planned Learning Activities and Teaching Methods
  • Lecture
  • Experiment
  • Report Preparation
  • Other
Assessment Methods and Criteria
Quantity Percentage (%)
Mid-term 1 25
Assignment 1 15
Quiz 10 10
Practice 10 10
Total(%) 60
Contribution of In-term Studies to Overall Grade(%) 60
Contribution of Final Examination to Overall Grade(%) 40
Total(%) 100
ECTS Allocated Based on Student Workload
Activities Quantity Duration (Hour) Total Workload (Hour)
Midterm exam 1 2 2
Preparation for Quiz 10 1 10
Individual or group work
Preparation for Final exam 1 30 30
Course hours 10 1 10
Preparation for Midterm exam 1 28 28
Laboratory (including preparation) 10 4 40
Final exam 1 2 2
Homework 1 16 16
Quiz 11 0.25 2.75
Report writing 10 10 100
Total Work Load240.75
Total Work Load / 30 (h)8.02
ECTS Credit of the Course8
Language of InstructionEnglish
Work Placement(s)No

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