TECHNIQUES IN IMMUNOHISTOCHEMISTRY

PLT210

Course Code: PLT210
Semester: Third Term (Fall)
Theory+Practice: 2 + 2
ECTS: 5

Course Type

Compulsory Course

Course Level

Associate Degree

Year of Study

Semester

Third Term (Fall)

ECTS

Name of Lecturer(s)

Assistant Professor Yasemin Sezgin

Learning Outcomes

At the end of this course, the students;

Understands the basic principles of immunohistochemistry and identifies the basic components of immunohistochemical technique.
Compares the techniques required for the preparation of a tissue before IHC staining and selects the appropriate one.
Knows and applies all components of immunohistochemical staining technique.
Accurately analyze immunohistochemical staining results and develop strategies to solve common problems.

Mode of Delivery

Face to face

Prerequisites

Recommended Optional Programme Components

None

Course Description

Course Scope and Objectives: This course provides a comprehensive understanding of Immunohistochemistry (IHC), focusing on its applications in pathology and diagnosis. The course begins with an introduction to basic immunology, where students learn the fundamentals of the immune response, including antigens, antibodies, epitopes, and their interactions. These fundamental concepts are critical to understanding the principles behind IHC. Next, students explore the different components of IHC, such as the types of antibodies (monoclonal and polyclonal), the use of primary and secondary antibodies, and their roles in detecting specific antigens in tissue samples. Antigen retrieval techniques, blocking nonspecific binding, and applying chromogenic and fluorescent staining methods are covered extensively. Through hands-on laboratory activities, students develop the skills needed to perform these procedures on formalin-fixed, paraffin-embedded (FFPE) tissue specimens. They also learn to develop strategies to overcome these challenges by troubleshooting common IHC problems such as poor staining, nonspecific binding, and high background.

Course Contents

WEEK	TOPICS
1^st Week	Introduction to the Course and Course Plan
2^nd Week	Introduction to Immunohistochemistry and Antigens
3^rd Week	Tumor Antigens and Oncogenes
4^th Week	Main Components of Immunohistochemical Techniques: Antibodies
5^th Week	Main Components of Immunohistochemical Techniques: Enzymes
6^th Week	Fixation and Antigen Retrieval
7^th Week	General Review of Fundamental Concepts in Immunohistochemistry
8^th Week	Midterm Examination
9^th Week	Staining Techniques
10^th Week	Controls in Immunohistochemistry
11^th Week	Interpretation of Immunohistochemical Results
12^th Week	Troubleshooting in Immunohistochemistry
13^th Week	Applications of Immunohistochemistry in Pathology
14^th Week	Advanced Techniques in Immunohistochemistry

Recommended or Required Reading

Histotechnology. Carson, F.L., Hladik, C., Cytopreparatory Techniques, ASCP, 2009.
Immunohistochemistry: Basics and Methods. Buchwalow, I.B., Beocker, W., Springer, 2010.
Diagnostic Immunohistochemistry. Dabbs, D.J., Churchill Livingstone, 2002.
İmmunoloji. Male, D.,Brostoff, J., Roth, D.B., Roitt, I., Palme Yayıncılık, 2009.

Planned Learning Activities and Teaching Methods

Lecture
Questions/Answers
Discussion
Presentation
Practice
Experiment
Problem Solving
Report Preparation
Case Study
Other

Assessment Methods and Criteria

	Quantity	Percentage (%)
Mid-term	1	30
Assignment	1	10
Quiz	1	10
Practice	1	10
Total(%)		60
Contribution of In-term Studies to Overall Grade(%)		60
Contribution of Final Examination to Overall Grade(%)		40
Total(%)		100

ECTS Allocated Based on Student Workload

Activities	Quantity	Duration (Hour)	Total Workload (Hour)
Midterm exam	1	1	1
Preparation for Quiz	1	5	5
Individual or group work	10	5	50
Preparation for Final exam	8	3	24
Course hours	14	2	28
Preparation for Midterm exam	5	2	10
Laboratory (including preparation)	14	2	28
Final exam	1	1	1
Homework	1	4	4
Total Work Load			151
Total Work Load / 30 (h)			5.03
ECTS Credit of the Course			5

Language of Instruction

Turkish

Work Placement(s)

Programme Qualifications / Learning Outcomes

	LO1	LO2	LO3	LO4
K1	X	X	X
K2
K3	X	X	X	X
K4	X
K5			X	X
K6		X	X	X
K7			X	X
K8				X
K9
K10
K11
K12			X
K13		X	X	X
K14	X	X	X	X
K15				X

	LO1	LO2	LO3	LO4
K1	X	X	X
K2
K3	X	X	X	X
K4	X
K5			X	X
K6		X	X	X
K7			X	X
K8				X
K9
K10
K11
K12			X
K13		X	X	X
K14	X	X	X	X
K15				X

	LO1	LO2	LO3	LO4
K1	X	X	X
K2
K3	X	X	X	X
K4	X
K5			X	X
K6		X	X	X
K7			X	X
K8				X
K9
K10
K11
K12			X
K13		X	X	X
K14	X	X	X	X
K15				X

	LO1	LO2	LO3	LO4
K1	X	X	X
K2
K3	X	X	X	X
K4	X
K5			X	X
K6		X	X	X
K7			X	X
K8				X
K9
K10
K11
K12			X
K13		X	X	X
K14	X	X	X	X
K15				X