| Course Type | Elective Course |
|---|
| Course Level | Master's Degree With Thesis |
|---|
| Year of Study | - |
|---|
| Semester | - |
|---|
| ECTS | 10 |
|---|
| Name of Lecturer(s) |
- |
| Learning Outcomes |
At the end of this course, the students;
- Understand the theory and principles of cellular toti-potency.
- Understand the principles behind single cell regeneration which is known as plant cell and tissue culture.
- Know the various methods or pathways by which single cells develop into a tissue, organ or whole plant.
- Understand the Genetic improvement at the single cell level and how this can be translated at the organism level.
- Know how to regenerate plants in sterile environment.
- Know how to use plant cell and tissue culture techniques for both research and commercial purposes.
|
| Mode of Delivery | Face to face |
|---|
| Prerequisites | No |
|---|
| Recommended Optional Programme Components | Plant Biology |
|---|
| Course Description | Principles and culture techniques of cells, callus, organs, pollen, anthers, embryos, and protoplasts. The applications in clonal propagation and research in breeding, physiology, and pathology. |
|---|
| Course Contents |
| WEEK |
TOPICS |
|
1st Week |
Tissue culture history, basis for plant tissue culture |
|
2nd Week |
Laboratory design and aseptic techniques |
|
3rd Week |
Nutrition of plant tissue cultures |
|
4th Week |
Dealing with biological contaminants |
|
5th Week |
Callus and cell suspension cultures |
|
6th Week |
Meristem culture and clonal propagation |
|
7th Week |
Somatic embryogenesis and synthetic seeds |
|
8th Week |
Direct and indirect organogenesis |
|
9th Week |
Anther culture, haploidy and embryo rescue |
|
10th Week |
Protoplast culture and somatic hybridization acclimatization and establishment of in vitro grown plants |
|
11th Week |
Paper presentation |
|
12th Week |
Paper presentation |
|
13th Week |
Paper presentation |
|
14th Week |
Paper presentation |
|
| Recommended or Required Reading | No text books are required as the lectures are compiled from a number of such books. |
|---|
| Planned Learning Activities and Teaching Methods |
- Lecture
- Discussion
- Questions/Answers
- Presentation
|
|---|
| Assessment Methods and Criteria |
|
Quantity |
Percentage (%) |
| Presentation of Article |
3 |
60 |
| Other |
1 |
40 |
| Total(%) |
|
100 |
| Contribution of In-term Studies to Overall Grade(%) |
|
100 |
| Contribution of Final Examination to Overall Grade(%) |
|
0 |
| Total(%) |
|
100 |
|
| ECTS Allocated Based on Student Workload |
| Activities |
Quantity |
Duration (Hour) |
Total Workload (Hour) |
| Midterm exam |
|
|
|
| Preparation for Quiz |
|
|
|
| Individual or group work |
|
|
|
| Preparation for Final exam |
1 |
105 |
105 |
| Course hours |
14 |
3 |
42 |
| Preparation for Midterm exam |
|
|
|
| Laboratory (including preparation) |
|
|
|
| Final exam |
1 |
3 |
3 |
| Homework |
|
|
|
| Article Presentation |
3 |
50 |
150 |
| Total Work Load | 300 |
| Total Work Load / 30 (h) | 10 |
| ECTS Credit of the Course | 10 |
|
| Language of Instruction | English |
|---|
| Work Placement(s) | No |
|---|
