IMMUNOLOGICAL TECHNIQUES

MBGE516

Course Code: MBGE516
Semester: -
Theory+Practice: 3 + 0
ECTS: 10

Course Type

Elective Course

Course Level

Master's Degree With Thesis

Year of Study

Semester

ECTS

Name of Lecturer(s)

Learning Outcomes

At the end of this course, the students;

Know the basic principles of innate and adaptive immune system.
Have a good knowledge of the basis for the structure, production and purification of monoclonal and polyclonal antibodies.
Have a good theoretical knowledge of methods like ELISA, immunoblotting, immunohistochemical staining techniques, direct and indirect immunofluorescence assays, flow cytometry, and immunoprecipitation assays.
Understand how immunological principles used in industry and research.
Gain experience in literature search, presentation and discussion of current research articles or reviews about immunological techniques.

Mode of Delivery

Face to face

Prerequisites

Recommended Optional Programme Components

Course Description

Immunological Techniques course gives information about polyclonal and monoclonal antibody generation and purification, detection of antigens in cells, tissues, fluids and solid supports using antibodies, direct and indirect immunofluorescence assays, flow cytometry, immunoassay of antigen by ELISA, immunoblotting, immunoprecipitation of antigen complexes, chromatin immunoprecipitation (ChIP) assays and also immunohistochemical staining techniques.

Course Contents

WEEK	TOPICS
1^st Week	Course introduction, basic principles of immunology
2^nd Week	Production and purification of antibodies
3^rd Week	Current literature presentation and discussion about monoclonal antibody technology
4^th Week	ELISA and immunoblotting
5^th Week	Current literature presentation and discussion
6^th Week	Immunohistochemical staining techniques
7^th Week	Current literature presentation and discussion
8^th Week	Immunoprecipitation assays
9^th Week	Direct and indirect immunofluorescence assays
10^th Week	Flow cytometry
11^th Week	Current literature presentation and discussion
12^th Week	Immunological principles used in industry and research
13^th Week	Research article presentation and discussion
14^th Week	Research article presentation and discussion

Recommended or Required Reading

Immunochemistry methods: Antigen and antibody production. Immunological tests, Tamara Krstic, 2015.
Immunoassay and Other Bioanalytical Techniques by Jeanette M. van Emon.
Immunoassays development, applications and future trends by Richard OKennedy, Caroline Murphy.
The Immunoassay Handbook. Theory and Applications of Ligand Binding, ELISA and Related Techniques by David Wild.
Current research articles and reviews

Planned Learning Activities and Teaching Methods

Lecture
Discussion
Questions/Answers
Presentation
Report Preparation

Assessment Methods and Criteria

	Quantity	Percentage (%)
Mid-term	1	25
Presentation of Article	5	40
Total(%)		65
Contribution of In-term Studies to Overall Grade(%)		65
Contribution of Final Examination to Overall Grade(%)		35
Total(%)		100

ECTS Allocated Based on Student Workload

Activities	Quantity	Duration (Hour)	Total Workload (Hour)
Midterm exam	1	3	3
Preparation for Quiz
Individual or group work
Preparation for Final exam	1	25	25
Course hours	14	3	42
Preparation for Midterm exam	1	20	20
Laboratory (including preparation)
Final exam
Homework
Article Presentation	5	42	210
Total Work Load			300
Total Work Load / 30 (h)			10
ECTS Credit of the Course			10

Language of Instruction

English

Work Placement(s)

Programme Qualifications / Learning Outcomes

	LO1	LO2	LO3	LO4	LO5
K1		X	X
K2		X	X
K3				X
K4					X
K5			X
K6				X	X
K7					X
K8		X	X	X