BIOANALYSIS: TECHNIQUES FOR CHARACTERIZATION OF BIOLOGICAL MATERIALS

MBGE513

Course Code: MBGE513
Semester: -
Theory+Practice: 3 + 0
ECTS: 10

Course Type

Elective Course

Course Level

Master's Degree With Thesis

Year of Study

Semester

ECTS

Name of Lecturer(s)

Assistant Professor Sema Zabcı

Learning Outcomes

At the end of this course, the students;

Define the basic principles of biological analysis.
Describe the techniques used for the isolation and separation of biomolecules.
Discuss the principles involved in the application of standard sample preparation procedures.
Explain the use of enzymes, antibodies, cells, and DNA in biosensors by detailing their specific roles and mechanisms.
Explain with examples how biological molecules are used in advanced technologies such as lab-on-a-chip, and discusses the analytical and practical advantages of such applications.

Mode of Delivery

Face to face

Prerequisites

Recommended Optional Programme Components

Biochemistry, Biomolecules, Biomaterials, Nanotechnology

Course Description

This course discusses important analytical methods for the characterization of biological macromolecules such as proteins, DNA, RNA, carbohydrates, and lipids. It also covers the use of enzymes, antibodies, cells, and DNA in emerging technologies and biosensors.

Course Contents

WEEK	TOPICS
1^st Week	Course overview
2^nd Week	Sample preparation and protein purification
3^rd Week	Protein determination
4^th Week	Enzymes and enzymatic analysis
5^th Week	Immunological techniques
6^th Week	Spectroscopy
7^th Week	Chromatographic separation methods and student presentations
8^th Week	Isolation and purification of nucleic acids
9^th Week	Analysis of nucleic acids
10^th Week	Analysis of carbohydrates
11^th Week	Analysis of lipids
12^th Week	Immobilized enzymes, biosensors and lab on a chip technology
13^th Week	3-D Structure determination (Magnetic resonance spectroscopy of biomolecules, electron microscopy, atomic force
14^th Week	microscopy)
15^th Week	Students presentations & discussions

Recommended or Required Reading

Bioanalytics: Analytical Methods and Concepts in Biochemistry and Molecular Biology, Friedrich Lottspeich, Joachim W. Engels, Wiley, 2018.
Principles and Practice of Bioanalysis, 2nd edition, Richard F. Venn, CRC Press, 2008.
Bioanalytical Chemistry, 2nd edition, Andreas Manz, Petra S Dittrich, Dimitri Iossifidis, 2015.
Biomolecular and Bioanalytical Techniques. Ramesh Vasudevan. Wiley Global Research (STMS); 2019.

Planned Learning Activities and Teaching Methods

Lecture
Discussion
Questions/Answers
Presentation
Other

Assessment Methods and Criteria

	Quantity	Percentage (%)
Mid-term	1	35
Presentation of Article	2	30
Total(%)		65
Contribution of In-term Studies to Overall Grade(%)		65
Contribution of Final Examination to Overall Grade(%)		35
Total(%)		100

ECTS Allocated Based on Student Workload

Activities	Quantity	Duration (Hour)	Total Workload (Hour)
Midterm exam	1	3	3
Preparation for Quiz
Individual or group work
Preparation for Final exam	1	90	90
Course hours	14	3	42
Preparation for Midterm exam	1	82	82
Laboratory (including preparation)
Final exam	1	3	3
Homework
Article Presentation	2	40	80
Total Work Load			300
Total Work Load / 30 (h)			10
ECTS Credit of the Course			10

Language of Instruction

English

Work Placement(s)

Programme Qualifications / Learning Outcomes

	LO1	LO2	LO3	LO4	LO5
K1		X	X
K2		X	X
K3	X	X	X	X	X
K4	X	X	X	X	X
K5
K6	X	X	X	X	X
K7	X	X	X	X	X
K8