Information Package

Master's of Molecular Biology and Genetics with Thesis (English)

MBGE505

ADVANCED RESEARCH TECHNIQUES IN MOLECULAR BIOLOGY

Course Code
MBGE505
Semester
-
Theory+Practice
3 + 0
ECTS
10
Course TypeElective Course
Course LevelMaster's Degree With Thesis
Year of Study-
Semester-
ECTS10
Name of Lecturer(s) -
Learning Outcomes

At the end of this course, the students;

  1. Learn advanced PCR based techniques designed for specific aims.
  2. Use of immunodetection and immunoseperation based analysis in molecular biology research.
  3. Gain knowledge of the use of high throughput metagenomic, genomic and transcriptomic analysis techniques, and their bioinformatics applications.
  4. Learn writing a project proposal and budget planning for proposals on varying research areas of molecular biology.
Mode of DeliveryFace to face
PrerequisitesNo
Recommended Optional Programme ComponentsMolecular Biology
Course DescriptionThis course is designed to teach the students the essential and advanced experimental design for molecular genetics studies, the details of molecular cloning, preparation and analyses of nucleic acids and proteins, analyses of recombinant organisms, genome editing and sequencing.
Course Contents
WEEK TOPICS
1st Week Introduction, academic writing: rules and principles
2nd Week Methylation-specific PCR (MSP)
3rd Week Telomerase repeated amplification protocol (TRAP) for telomerase activity
4th Week Digital droplet PCR and applications (ddPCR)
5th Week Flow cytometry based analysis
6th Week Immunoprecipitation for protein interactions
7th Week Student presentations
8th Week Microarray analysis
9th Week Metagenomic studies and bioinformatic applications
10th Week Writing a project proposal for MBG research I
11th Week Writing a project proposal for MBG research II
12th Week Budget planning for MBG research proposals
13th Week Discussion on student reviews / projects
14th Week Student presentations and discussion on articles
Recommended or Required ReadingArticles, web sources, Current Protocols in Molecular Biology, Wiley Online Library.
Planned Learning Activities and Teaching Methods
  • Lecture
  • Discussion
  • Presentation
  • Project
Assessment Methods and Criteria
Quantity Percentage (%)
Project 1 55
Presentation of Article 1 25
Presentation of Case Study 1 5
Other 1 15
Total(%) 100
Contribution of In-term Studies to Overall Grade(%) 100
Contribution of Final Examination to Overall Grade(%) 0
Total(%) 100
ECTS Allocated Based on Student Workload
Activities Quantity Duration (Hour) Total Workload (Hour)
Midterm exam
Preparation for Quiz
Individual or group work
Preparation for Final exam
Course hours 14 3 42
Preparation for Midterm exam
Laboratory (including preparation)
Final exam
Homework
Presentation (including preperation) 1 55 55
Project 1 150 150
Article Presentation 1 55 55
Total Work Load302
Total Work Load / 30 (h)10.06
ECTS Credit of the Course10
Language of InstructionEnglish
Work Placement(s)No
LO1 LO2 LO3 LO4
K1 X
K2 X X X X
K3 X X X X
K4 X
K5 X X
K6 X
K7 X
K8 X