| Course Type | Elective Course |
|---|
| Course Level | Doctorate Of Science |
|---|
| Year of Study | - |
|---|
| Semester | - |
|---|
| ECTS | 8 |
|---|
| Name of Lecturer(s) |
- |
| Learning Outcomes |
At the end of this course, the students;
- Recognizes devices and materials used in molecular microbiology laboratories.
- Knows the definitions and mechanisms of microorganism lysis and nucleic acid purification methods.
- List the principles of nucleic acid amplification and agarose gel electrophoresis of amplified gene products.
- Defines molecular typing and epidemiology methods.
|
| Mode of Delivery | Face to face |
|---|
| Prerequisites | No |
|---|
| Recommended Optional Programme Components | - |
|---|
| Course Description | It is aimed to identify the devices and materials used in molecular microbiology laboratories, to define and apply microorganism lysis and nucleic acid purification methods, to learn the principles of nucleic acid amplification and to show amplified gene products in agarose gel electrophoresis, to define molecular typing and epidemiology methods. |
|---|
| Course Contents |
| WEEK |
TOPICS |
|
1st Week |
Introduction and rules of molecular microbiology laboratory |
|
2nd Week |
Methods used in molecular microbiology |
|
3rd Week |
Nucleic acid extraction and polimerase chain reaction |
|
4th Week |
Electrophoresis and amplification imaging systems |
|
5th Week |
Nucleic acid extraction and polimerase chain reaction-practical |
|
6th Week |
Electrophoresis and amplification imaging systems-practical |
|
7th Week |
Nucleic acid hybridization methods |
|
8th Week |
Nucleic acid amplification methods |
|
9th Week |
Nucleic acid sequence analysis methods |
|
10th Week |
Nucleic acid sequence analysis methods |
|
| Recommended or Required Reading | 1. Molecular Microbiology, Diagnostic Principles and Practice. (Eds: Persing DH, Tenover FC, Versalovic J, Tang Y, Unger ER, Relman DA, White TJ). ASM Press, Washington DC, 2004.
2. Molecular Cloning, A Laboratory Manual. (Eds: Sambrook J, Russell DW). Cold Spring Harbor Laboratory Press, New York, 2001.
3. Real-Time PCR, An Essential Guide. (Eds: Edwards K, Logan J, Saunders N). Horizon Bioscience, Norfolk, UK, 2004.
|
|---|
| Planned Learning Activities and Teaching Methods |
- Lecture
- Discussion
- Questions/Answers
- Practice
|
|---|
| Assessment Methods and Criteria |
|
Quantity |
Percentage (%) |
| Mid-term |
1 |
40 |
| Total(%) |
|
40 |
| Contribution of In-term Studies to Overall Grade(%) |
|
40 |
| Contribution of Final Examination to Overall Grade(%) |
|
60 |
| Total(%) |
|
100 |
|
| ECTS Allocated Based on Student Workload |
| Activities |
Quantity |
Duration (Hour) |
Total Workload (Hour) |
| Midterm exam |
1 |
1 |
1 |
| Preparation for Quiz |
0 |
0 |
0 |
| Individual or group work |
14 |
10 |
140 |
| Preparation for Final exam |
1 |
21 |
21 |
| Course hours |
14 |
2 |
28 |
| Preparation for Midterm exam |
1 |
20 |
20 |
| Laboratory (including preparation) |
14 |
2 |
28 |
| Final exam |
1 |
2 |
2 |
| Homework |
0 |
0 |
0 |
| Total Work Load | 240 |
| Total Work Load / 30 (h) | 8 |
| ECTS Credit of the Course | 8 |
|
| Language of Instruction | Turkish |
|---|
| Work Placement(s) | No |
|---|
