| Course Type | Elective Course |
|---|
| Course Level | Bachelor's Degree |
|---|
| Year of Study | - |
|---|
| Semester | - |
|---|
| ECTS | 4 |
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| Name of Lecturer(s) |
|
| Learning Outcomes |
At the end of this course, the students;
- Understand the principles of microscopic, spectroscopic, chromatographic, electrophoretic, and immunoassay-based bioanalytical techniques.
- Apply these techniques to analyze biomolecules (proteins, nucleic acids, metabolites).
- Differentiate between bioanalytical techniques in terms of their principles, sensitivity, resolution, and suitability for analyzing different biomolecules.
- Interpret data generated from techniques
- Select and justify the most appropriate analytical method for specific biological questions and evaluate its applications and limitations in research, clinical, and industrial contexts.
|
| Mode of Delivery | Face to face |
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| Prerequisites | No |
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| Recommended Optional Programme Components | No |
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| Course Description | This course introduces fundamental and advanced bioanalytical techniques used in molecular biology research and diagnostics. It covers spectroscopic, chromatographic, electrophoretic, and immunoassay-based methods, along with real-world applications in biological and clinical research. |
|---|
| Course Contents |
| WEEK |
TOPICS |
|
1st Week |
Introduction to Bioanalytical Techniques |
|
2nd Week |
Sample preparation techniques: Filtration, Centrifugation, Extraction ? Buffer Solutions |
|
3rd Week |
Microscopic techniques I: Light and fluorescence microscopes |
|
4th Week |
Microscopic techniques II:Electron microscopes |
|
5th Week |
Imaging Live Cells and Tissues, Measuring Cellular Dynamics |
|
6th Week |
Chromatographic techniques I? LC, GC, Supercritical Fluid, Adsorption, Paper Chromatography, |
|
7th Week |
Chromatographic techniques II? Affinity, Ion Exchange, Size Exclusion Chromatography, HPLC |
|
8th Week |
Flow Cytometer (Fluidic and optical systems, analyses, functional assays) |
|
9th Week |
Immunochemical Techniques I (ELISA, Western Blot, Immunoprecipitation ) |
|
10th Week |
Immunochemical Techniques II (FACS, biosensors) |
|
11th Week |
Spectroscopic Techniques I (UV Visible, Infrared, Raman, Fluorescence Spectroscopy) |
|
12th Week |
Spectroscopic Techniques II (NMR, Circular Dichroism, Atomic, X-ray Spectroscopy) |
|
13th Week |
Mass spectrometry |
|
14th Week |
Discussion |
|
| Recommended or Required Reading | Hofmann, A., & Clokie, S. (Eds.). (2018). Wilson and Walker?s Principles and Techniques of Biochemistry and Molecular Biology (8th ed.). Cambridge: Cambridge University Press.
Principles and techniques of biochemistry and molecular biology. K. Wilson, J. M. Walker. Cambridge University Press. 7th ed., 2009. |
|---|
| Planned Learning Activities and Teaching Methods |
- Lecture
- Discussion
- Questions/Answers
|
|---|
| Assessment Methods and Criteria |
|
Quantity |
Percentage (%) |
| Mid-term |
1 |
40 |
| Quiz |
1 |
10 |
| Presentation of Article |
1 |
10 |
| Total(%) |
|
60 |
| Contribution of In-term Studies to Overall Grade(%) |
|
60 |
| Contribution of Final Examination to Overall Grade(%) |
|
40 |
| Total(%) |
|
100 |
|
| ECTS Allocated Based on Student Workload |
| Activities |
Quantity |
Duration (Hour) |
Total Workload (Hour) |
| Midterm exam |
1 |
2 |
2 |
| Preparation for Quiz |
1 |
10 |
10 |
| Individual or group work |
|
|
|
| Preparation for Final exam |
1 |
35 |
35 |
| Course hours |
14 |
3 |
42 |
| Preparation for Midterm exam |
1 |
30 |
30 |
| Laboratory (including preparation) |
|
|
|
| Final exam |
1 |
2 |
2 |
| Homework |
|
|
|
| Quiz |
1 |
0.5 |
0.5 |
| Article Presentation |
1 |
0.5 |
0.5 |
| Total Work Load | 122 |
| Total Work Load / 30 (h) | 4.06 |
| ECTS Credit of the Course | 4 |
|
| Language of Instruction | English |
|---|
| Work Placement(s) | No |
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