- Course Code
- MBGE409
- Semester
- Sixth Term (Spring)
- Theory+Practice
- 2 + 2
- ECTS
- 5
| Course Type | Compulsory Course |
|---|
| Course Level | Bachelor's Degree |
|---|
| Year of Study | 3 |
|---|
| Semester | Sixth Term (Spring) |
|---|
| ECTS | 5 |
|---|
| Name of Lecturer(s) |
|
| Learning Outcomes |
At the end of this course, the students;
- Know the infrastructure of animal tissue culture, basic safety and aseptic techniques, and standard cultures.
- Know primary tissue culture, suspension culture and monolayer culture, freeze and thaw the cell lines.
- Know the infrastructure of plant tissue culture, seed and plant sterilization, and different culture media.
- Know the subculturing, direct and indirect organogenesis.
- Know all the laboratory applications studied during the course and can interpret the experiment results.
|
| Mode of Delivery | Face to face |
|---|
| Prerequisites | No |
|---|
| Recommended Optional Programme Components | None |
|---|
| Course Description | In this course, students learn the culturing methods of animal and plant cells both theoretically and practically and have knowledge about the application areas of plant-animal tissue culture. |
|---|
| Course Contents |
| WEEK |
TOPICS |
|
1st Week |
Course Introduction |
|
2nd Week |
Animal Tissue Culture Laboratory (Design and Equipment) / Sterilization and Basic Aseptic Techniques |
|
3rd Week |
Animal Tissue Culture Media / Primary Tissue Culture / Cell Lines |
|
4th Week |
Suspension & Monolayer Cultures / 3D Cell Cultures |
|
5th Week |
Mobility & Migration Assays, Cytotoxicity & Proliferation Assays Cell Storage (Cell Freezing and Thawing) |
|
6th Week |
Plant Tissue Culture Laboratory (History, Design and Equipment) Seed and Plant Sterilization / Plant Tissue Culture Media |
|
7th Week |
Subculturing / Direct Organogenesis / Indirect Organogenesis |
|
8th Week |
Acclimatization and Establishment of In Vitro Grown Plants, Biotechnological Purposes and Large Scale Production, Secondary Metabolite Production |
|
9th Week |
Animal Tissue Culture Laboratory: Learning Aseptic Working Conditions, Animal Tissue Culture Medium Preparation, Cell Thawing |
|
10th Week |
Cell Medium Change and Cell Passaging |
|
11th Week |
Cell Freezing |
|
12th Week |
Plant Tissue Culture Laboratory: Medium Preparation/ Seed Sterilization and Planting/Leaf Surface Sterilization and Planting |
|
13th Week |
Callus Culture and Subculturing |
|
14th Week |
Course Overview |
|
| Recommended or Required Reading | Culture of Animal Cells: A Manual of Basic Techniques, 6th ed. R. Ian Freshney. John Wiley & Sons, Inc., 2010.
Plant Tissue Culture Techniques and Experiments, Roberta H. Smith, Third Edition, 2012. |
|---|
| Planned Learning Activities and Teaching Methods |
- Lecture
- Questions/Answers
- Experiment
- Report Preparation
|
|---|
| Assessment Methods and Criteria |
|
Quantity |
Percentage (%) |
| Mid-term |
1 |
35 |
| Practice |
1 |
25 |
| Total(%) |
|
60 |
| Contribution of In-term Studies to Overall Grade(%) |
|
60 |
| Contribution of Final Examination to Overall Grade(%) |
|
40 |
| Total(%) |
|
100 |
|
| ECTS Allocated Based on Student Workload |
| Activities |
Quantity |
Duration (Hour) |
Total Workload (Hour) |
| Midterm exam |
1 |
2 |
2 |
| Preparation for Quiz |
4 |
3 |
12 |
| Individual or group work |
|
|
|
| Preparation for Final exam |
1 |
33 |
33 |
| Course hours |
14 |
2 |
28 |
| Preparation for Midterm exam |
1 |
28 |
28 |
| Laboratory (including preparation) |
13 |
2 |
26 |
| Final exam |
1 |
2 |
2 |
| Homework |
1 |
8 |
8 |
| Quiz |
4 |
0.5 |
2 |
| Report writing |
1 |
10 |
10 |
| Total Work Load | 151 |
| Total Work Load / 30 (h) | 5.03 |
| ECTS Credit of the Course | 5 |
|
| Language of Instruction | English |
|---|
| Work Placement(s) | No |
|---|
Programme Qualifications / Learning Outcomes
|
LO1 |
LO2 |
LO3 |
LO4 |
LO5 |
| K1 |
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|
|
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| K2 |
X |
X |
X |
X |
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| K3 |
X |
X |
X |
X |
|
| K4 |
|
|
|
|
|
| K5 |
|
|
|
|
|
| K6 |
X |
X |
X |
X |
X |
| K7 |
|
|
|
|
X |
| K8 |
|
|
|
|
X |
| K9 |
|
|
|
|
X |
| K10 |
X |
X |
X |
X |
X |
| K11 |
|
|
|
|
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| K12 |
|
|
|
|
X |
| K13 |
|
|
|
|
X |
