Information Package
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Program of Molecular Biology and Genetics (English)

MBGE305

REKOMBINANT DNA TECHNOLOGY

Course Code
MBGE305
Semester
Fifth Term (Fall)
Theory+Practice
3 + 0
ECTS
4
Course TypeCompulsory Course
Course LevelBachelor's Degree
Year of Study3
SemesterFifth Term (Fall)
ECTS4
Name of Lecturer(s)
Learning Outcomes

At the end of this course, the students;

  1. Explain timeline of recombinant DNA technology, fundamentals of molecular cloning and vectors.
  2. Distinguish transformation and transfection techniques.
  3. Suggest methodologies for genome and cDNA library preparation and screening.
  4. Design recombinant protein production in different cell types.
  5. Comment on fundamentals of custom vector design and techniques for specific purposes.
Mode of DeliveryFace to face
PrerequisitesNo
Recommended Optional Programme ComponentsNo
Course DescriptionIn this course, basic principles of molecular cloning, screening of clones, determination of target gene expression, genome and cDNA libraries, and recombinant protein production are given.
Course Contents
WEEK TOPICS
1st Week Introduction to Recombinant DNA Technology
2nd Week Blotting and Hybridization Based Techniques for Screening
3rd Week DNA Isolation and Vectors
4th Week Vectors II
5th Week PCR Based Techniques
6th Week October 29 Week
7th Week Repeat Before Midterm
8th Week Sequencing Techniques
9th Week Cutting and Joining DNA Molecules
10th Week Gene Transfer
11th Week Gene Transfer in Medicine
12th Week Genomic and cDNA Libraries
13th Week Recombinant Protein Production
14th Week Discussion and Repeat
Recommended or Required ReadingPrinciples of Gene Manipulation and Genomics. Sandy B. Primrose, Richard Twyman, 7th edition, 2006.
Molecular Biotechnology, Principles and Applications of Recombinant DNA. Bernard R. Glick, Jack J. Pasternak, ASM Press, Washington, D.C., 2009.
Planned Learning Activities and Teaching Methods
  • Lecture
  • Questions/Answers
  • Presentation
  • Other
Assessment Methods and Criteria
Quantity Percentage (%)
Mid-term 1 35
Assignment 1 15
Total(%) 50
Contribution of In-term Studies to Overall Grade(%) 50
Contribution of Final Examination to Overall Grade(%) 50
Total(%) 100
ECTS Allocated Based on Student Workload
Activities Quantity Duration (Hour) Total Workload (Hour)
Midterm exam 1 2 2
Preparation for Quiz
Individual or group work
Preparation for Final exam 1 30 30
Course hours 14 3 42
Preparation for Midterm exam 1 25 25
Laboratory (including preparation)
Final exam 1 2 2
Homework 1 20 20
Total Work Load121
Total Work Load / 30 (h)4.03
ECTS Credit of the Course4
Language of InstructionEnglish
Work Placement(s)No

Programme Qualifications / Learning Outcomes

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