Apply isolation methods for DNA, RNA and plasmid from the cell, and quantify these isolates, evaltuate the purity of samples.
Apply basic genetic studies using Drosophila melanogaster, explain the basic terms related to patters of inheritance.
Amplify a gene by PCR method and knows, evaluates, and reports other uses of PCR in life and health sciences.
Determine, analyze and discuss the expression level of a gene.
Mode of Delivery
Face to face
Prerequisites
No
Recommended Optional Programme Components
No
Course Description
This course aim to enable students with learning of techniques such as DNA, RNA, plasmid DNA isolation, RT-PCR, gel electropheresis used in molecular biology, and practical information about these techniques.
Course Contents
WEEK
TOPICS
1st Week
Introduction to laboratory
2nd Week
DNA isolation from blood
3rd Week
Plasmid isolation
4th Week
Spectrophotometric measurment for nucleic acid quantitation
5th Week
Agarose gel electrophoresis
6th Week
PCR
7th Week
Restriction enzyme digestion
8th Week
RAPD
9th Week
RFLP
10th Week
RNA isolation
11th Week
RT-PCR
12th Week
Patterns of Inheritance
13th Week
Make-up week
14th Week
Make-up week
Recommended or Required Reading
Laboratory manual
Planned Learning Activities and Teaching Methods
Experiment
Report Preparation
Lecture
Assessment Methods and Criteria
Quantity
Percentage (%)
Mid-term
1
30
Quiz
10
10
Other
11
20
Total(%)
60
Contribution of In-term Studies to Overall Grade(%)
60
Contribution of Final Examination to Overall Grade(%)
