| Course Type | Elective Course |
|---|
| Course Level | Master's Degree With Thesis |
|---|
| Year of Study | - |
|---|
| Semester | - |
|---|
| ECTS | 10 |
|---|
| Name of Lecturer(s) |
- |
| Learning Outcomes |
At the end of this course, the students;
- Learns What Affinity is
- Learn What Affinity Chromatography Is
- May Link Affinity and Biotechnology
- Understanding the Connection Between Molecular Biology and Affinity Techniques can learn to use
|
| Mode of Delivery | |
|---|
| Prerequisites | No |
|---|
| Recommended Optional Programme Components | |
|---|
| Course Description | Within the scope of this course, Affinity and Affinity Chromatography, the Connection Between Affinity and Biotechnology, and the Connection Between Molecular Biology and Affinity Techniques are explained. |
|---|
| Course Contents |
| WEEK |
TOPICS |
|
1st Week |
Definition of Affinity Chromatography, Development and use of Affinity Chromatography, |
|
2nd Week |
Matrix selection in Affinity Chromatography |
|
3rd Week |
Ligand selection in Affinity Chromatography |
|
4th Week |
Affinity techniques used in molecular biology |
|
5th Week |
Cell purification by affinity chromatography |
|
6th Week |
Molecularly imprinted polymers and biochromatography applications |
|
7th Week |
midterm exam |
|
8th Week |
Immobilized Metal ion affinity chromatography |
|
9th Week |
Immunaffinte chromatography |
|
10th Week |
Dye-ligand chromatography |
|
11th Week |
monoliths |
|
12th Week |
Affinity precipitation of proteins using metal-chelate |
|
13th Week |
Purification of proteins by Gel Filtration and Ion exchange chromatography methods |
|
14th Week |
Lectin Affinity Chromatography |
|
| Recommended or Required Reading | Affinity Chromatography, C. Christopher R. Lowe, P. D. Peter D. G. Dean John Wiley & Sons, 1974 |
|---|
| Planned Learning Activities and Teaching Methods |
- Lecture
- Discussion
- Questions/Answers
|
|---|
| Assessment Methods and Criteria |
|
| ECTS Allocated Based on Student Workload |
| Activities |
Quantity |
Duration (Hour) |
Total Workload (Hour) |
| Midterm exam |
1 |
84 |
84 |
| Preparation for Quiz |
1 |
16 |
16 |
| Individual or group work |
0 |
0 |
0 |
| Preparation for Final exam |
1 |
24 |
24 |
| Course hours |
14 |
3 |
42 |
| Preparation for Midterm exam |
1 |
11 |
11 |
| Laboratory (including preparation) |
0 |
0 |
0 |
| Final exam |
1 |
96 |
96 |
| Homework |
1 |
12 |
12 |
| Project |
1 |
12 |
12 |
| Total Work Load | 297 |
| Total Work Load / 30 (h) | 9.9 |
| ECTS Credit of the Course | 10 |
|
| Language of Instruction | - |
|---|
| Work Placement(s) | No |
|---|
