| Course Type | Elective Course |
|---|
| Course Level | Master's Degree With Thesis |
|---|
| Year of Study | - |
|---|
| Semester | - |
|---|
| ECTS | 10 |
|---|
| Name of Lecturer(s) |
- |
| Learning Outcomes |
At the end of this course, the students;
- Learn basic animal cell culture laboratory infrastructure, sterilization and aseptic techniques, and medium types.
- Learn fundamentals of primary, suspension and monolayer cultures and passaging, cell storage techniques, and establishment of immortal cultures.
- Perform laboratory applications of the course content, and discuss experimental results.
|
| Mode of Delivery | Face to face |
|---|
| Prerequisites | No |
|---|
| Recommended Optional Programme Components | No |
|---|
| Course Description | Within the scope of this course; Animal tissue culture laboratory infrastructure, basic sterilization and aseptic techniques and culture media; primary tissue culture, suspension and monolayer cultures and passages, cell storage techniques and basics of cell transformation techniques are explained. |
|---|
| Course Contents |
| WEEK |
TOPICS |
|
1st Week |
Plant Cell Culture Laboratory |
|
2nd Week |
Sterilization and Aseptic Techniques in Plant Cell Culture |
|
3rd Week |
Sterilization of Seed and Plant |
|
4th Week |
Media Preparation |
|
5th Week |
Applications and Discussions |
|
6th Week |
Sub-culture |
|
7th Week |
Applications and Discussions |
|
8th Week |
MIDTERM |
|
9th Week |
Callus Culture |
|
10th Week |
Direct Organogenesis |
|
11th Week |
Applications and Discussions |
|
12th Week |
Indirect Organogenesis |
|
13th Week |
Applications and Discussions |
|
14th Week |
Presentations |
|
| Recommended or Required Reading | Culture of Animal Cells: A Manual of Basic Techniques, 6th ed. R. Ian Freshney. John Wiley & Sons, Inc., 2010.
Plant Tissue Culture Techniques and Experiments, Roberta H. Smith, Third Edition, 2012
|
|---|
| Planned Learning Activities and Teaching Methods |
- Lecture
- Discussion
- Questions/Answers
- Presentation
|
|---|
| Assessment Methods and Criteria |
|
Quantity |
Percentage (%) |
| Mid-term |
1 |
30 |
| Practice |
1 |
30 |
| Total(%) |
|
60 |
| Contribution of In-term Studies to Overall Grade(%) |
|
60 |
| Contribution of Final Examination to Overall Grade(%) |
|
40 |
| Total(%) |
|
100 |
|
| ECTS Allocated Based on Student Workload |
| Activities |
Quantity |
Duration (Hour) |
Total Workload (Hour) |
| Midterm exam |
1 |
84 |
84 |
| Preparation for Quiz |
1 |
16 |
16 |
| Individual or group work |
0 |
0 |
0 |
| Preparation for Final exam |
1 |
24 |
24 |
| Course hours |
14 |
3 |
42 |
| Preparation for Midterm exam |
1 |
11 |
11 |
| Laboratory (including preparation) |
0 |
0 |
0 |
| Final exam |
1 |
96 |
96 |
| Homework |
1 |
12 |
12 |
| Project |
1 |
12 |
12 |
| Total Work Load | 297 |
| Total Work Load / 30 (h) | 9.9 |
| ECTS Credit of the Course | 10 |
|
| Language of Instruction | Turkish |
|---|
| Work Placement(s) | No |
|---|
