Information Package
baskent.edu.tr

Master's of Biotechnology with Thesis

BTE502

LABORATORY PRACTICES IN BIOTECHNOLOGY

Course Code
BTE502
Semester
Second Term (Spring)
Theory+Practice
3 + 0
ECTS
10
Course TypeCompulsory Course
Course LevelMaster's Degree With Thesis
Year of Study1
SemesterSecond Term (Spring)
ECTS10
Name of Lecturer(s)
Learning Outcomes

At the end of this course, the students;

  1. Learn Fundamentals of Sterilization and Bacterial Cultures.
  2. Learn nucleic acid and protein isolation techniques, qualitative analysis by electrophoresis, and spectrophotometric quantitative analysis
  3. Learn fundamental techniques for molecular cloning of a target gene to a bacterial cell and screening of clones.
  4. Learn experimental analyses with obtained recombinant organisms.
  5. Learn the expression analysis via real-time quantitative RT-PCR.
Mode of DeliveryFace to face
PrerequisitesNo
Recommended Optional Programme ComponentsNo
Course Description
Course Contents
WEEK TOPICS
1st Week Fundamentals of Sterilization and Bacterial Cultures
2nd Week Fundamentals of Spectrophotometric Analysis
3rd Week DNA Isolation and Quantitation
4th Week Plasmid Isolation and Quantitation
5th Week Visualization of Nucleic Acids by Agarose Gel Electrophoresis
6th Week Protein Isolation and Quantitation
7th Week Visualization of Nucleic Acids by Polyacrylamide Gel Electrophoresis
8th Week Midterm
9th Week Polymerase Chain Reaction
10th Week Restriction Endonuclease Digestion
11th Week Vectors and Ligation
12th Week Competent Bacteria and Transformation
13th Week Screening of Clones
14th Week Student presentations
Recommended or Required Reading1. Moleküler Biyolojide Kullanılan Yöntemler, Prof. Dr. Güler Temizkan, 2004.
2. Molecular Cloning: A Laboratory Manual Michael R. Green,? Joseph Sambrook (Fourth Edition), 2012.
3. Current Protocols in Molecular Biology, Wiley Online Library.
Planned Learning Activities and Teaching Methods
  • Lecture
  • Discussion
  • Questions/Answers
  • Experiment
  • Report Preparation
  • Presentation
Assessment Methods and Criteria
Quantity Percentage (%)
Mid-term 1 20
Assignment 1 35
Total(%) 55
Contribution of In-term Studies to Overall Grade(%) 55
Contribution of Final Examination to Overall Grade(%) 45
Total(%) 100
ECTS Allocated Based on Student Workload
Activities Quantity Duration (Hour) Total Workload (Hour)
Midterm exam 1 84 84
Preparation for Quiz 1 16 16
Individual or group work 0 0 0
Preparation for Final exam 1 24 24
Course hours 14 3 42
Preparation for Midterm exam 1 11 11
Laboratory (including preparation) 0 0 0
Final exam 1 96 96
Homework 1 12 12
Project 1 12 12
Total Work Load297
Total Work Load / 30 (h)9.9
ECTS Credit of the Course10
Language of InstructionTurkish
Work Placement(s)No

Programme Qualifications / Learning Outcomes

LO1 LO2 LO3 LO4 LO5
K1 X X X X
K2 X X X X
K3 X X X X
K4
K5 X X X X
K6 X
K7 X X X X
K8
K9 X