NUCLEIC ACID AND PROTEIN CHEMISTRY

BTE501

Course Code: BTE501
Semester: First Term (Fall)
Theory+Practice: 3 + 0
ECTS: 10

Course Type

Compulsory Course

Course Level

Master's Degree With Thesis

Year of Study

Semester

First Term (Fall)

ECTS

Name of Lecturer(s)

Learning Outcomes

At the end of this course, the students;

Learns bonding structures of organic molecules.
Learns molecular structures and functions of nucleic acids and proteins.
Understands physiology of different cell types while evaluating structural information in terms of molecular evolution.
Learn to use structural knowledge for cellular modifications.

Mode of Delivery

Prerequisites

Recommended Optional Programme Components

Course Description

The aim of this course is to make students to learn and discuss the bonding structures of organic molecules, the structure of nucleic acids and protein molecules, Watson-Crick model of DNA, structure and types of RNA, transcription, translation, 3-D structure of proteins; to understand and discuss the methods for investigating nucleic acids and proteins such as Scanning Force Microscopy (SFM), High Pressure Liquid Chromatography (HPLC), Isoelectric Focusing (IeF), 3D Gel Electrophoresis, SDS Denaturation, Bradford Test. This course aims to provide students with a biological perspective. At the end of the course the students are also expected to understand physiology of different cell types while evaluating structural information in terms of molecular evolution and to use structural knowledge for cellular modifications.

Course Contents

WEEK	TOPICS
1^st Week	Introduction
2^nd Week	Atoms, molecules and their interactions
3^rd Week	Building blocks of DNA: nucleic acids
4^th Week	Physical and chemical properties of nucleic acids
5^th Week	Physical and chemical properties of proteins
6^th Week	Watson and Crick's model of DNA
7^th Week	Midterm Exam
8^th Week	Highly organized structure of DNA
9^th Week	Structure of RNA
10^th Week	Types of RNA
11^th Week	Transcription and Translation
12^th Week	Amino acids and 3D-structures of proteins
13^th Week	Examination methods of nucleic acids and proteins (SFM, HPLC, IeF, 3D Gel Electrophoresis, SDS Denaturing Gel, Bradford Protein Assay)
14^th Week	Project Presentation - Final Examination

Recommended or Required Reading

Lehninger Principles of Biochemistry 7th Edition, Albert L. Lehninger ve David L. Nelson

Planned Learning Activities and Teaching Methods

Lecture
Discussion
Questions/Answers
Project

Assessment Methods and Criteria

	Quantity	Percentage (%)
Mid-term	1	20
Project	1	30
Attendance	1	20
Total(%)		70
Contribution of In-term Studies to Overall Grade(%)		70
Contribution of Final Examination to Overall Grade(%)		30
Total(%)		100

ECTS Allocated Based on Student Workload

Activities	Quantity	Duration (Hour)	Total Workload (Hour)
Midterm exam	1	84	84
Preparation for Quiz	1	16	16
Individual or group work	0	0	0
Preparation for Final exam	1	24	24
Course hours	14	3	42
Preparation for Midterm exam	1	11	11
Laboratory (including preparation)	0	0	0
Final exam	1	96	96
Homework	1	12	12
Project	1	12	12
Total Work Load			297
Total Work Load / 30 (h)			9.9
ECTS Credit of the Course			10

Language of Instruction

Work Placement(s)

Programme Qualifications / Learning Outcomes

	LO1	LO2	LO3	LO4
K1	X	X	X
K2	X	X	X
K3	X	X	X
K4	X	X
K5	X	X	X
K6			X
K7	X	X	X
K8
K9	X	X	X