| Course Type | Elective Course |
|---|
| Course Level | Bachelor's Degree |
|---|
| Year of Study | - |
|---|
| Semester | - |
|---|
| ECTS | 4 |
|---|
| Name of Lecturer(s) |
|
| Learning Outcomes |
At the end of this course, the students;
- Defines the principles of bioanalytical techniques based on microscopic, spectroscopic,chromatographic, electrophoretic, and immunological analyses.
- Defines which biomolecules (proteins, nucleic acids, metabolites) these techniques are applied to analyze.
- Discusses the applications of these techniques in research and industry.
|
| Mode of Delivery | Face to face |
|---|
| Prerequisites | No |
|---|
| Recommended Optional Programme Components | No |
|---|
| Course Description | This course introduces the fundamental and advanced bioanalytical techniques used in molecular biology research. It covers spectroscopic, chromatographic, electrophoretic, and immunological analysis-based methods, as well as their applications in biological and clinical research. |
|---|
| Course Contents |
| WEEK |
TOPICS |
|
1st Week |
Introduction to Bioanalytical Techniques |
|
2nd Week |
Sample Preparation Techniques: Filtration, Centrifugation, Extraction-Buffer Solutions |
|
3rd Week |
Microscopic Techniques I: Light Microscopes |
|
4th Week |
Microscopic Techniques II: Electron Microscopes |
|
5th Week |
Chromatographic Techniques I -LC, GC, Supercritical Fluid, Adsorption, Paper Chromatography |
|
6th Week |
Chromatographic Techniques II- Affinity, Ion Exchange, Size Exclusion Chromatography, HPLC |
|
7th Week |
Flow Cytometry (Fluidic and Optical Systems, Analyses, Functional Analyses) |
|
8th Week |
Immunochemical Techniques I: ELISA, Western Blot, Immunoprecipitation |
|
9th Week |
Immunochemical Techniques II: FACS, Biosensors |
|
10th Week |
Spectroscopic Techniques I: UV-Visible, Infrared, Raman, Fluorescence Spectroscopy |
|
11th Week |
Spectroscopic Techniques II: NMR, Circular Dichroism, Atomic, X-ray Spectroscopy |
|
12th Week |
Mass Spectrometry I- Principles, Molecular Techniques Used in Bioanalysis |
|
13th Week |
Presentations |
|
14th Week |
Presentations-Quiz |
|
| Recommended or Required Reading | Principles and techniques of biochemistry and molecular biology. K. Wilson, J. M. Walker. Cambridge University Press. 7th ed., 2009.
Bioanalytical Chemistry. Susan R. Mikkelsen, Eduardo Corton. Wiley & Sons Press., 2004. |
|---|
| Planned Learning Activities and Teaching Methods |
- Lecture
- Discussion
- Questions/Answers
|
|---|
| Assessment Methods and Criteria |
|
Quantity |
Percentage (%) |
| Mid-term |
1 |
40 |
| Quiz |
1 |
10 |
| Presentation of Article |
1 |
10 |
| Total(%) |
|
60 |
| Contribution of In-term Studies to Overall Grade(%) |
|
60 |
| Contribution of Final Examination to Overall Grade(%) |
|
40 |
| Total(%) |
|
100 |
|
| ECTS Allocated Based on Student Workload |
| Activities |
Quantity |
Duration (Hour) |
Total Workload (Hour) |
| Midterm exam |
1 |
2 |
2 |
| Preparation for Quiz |
1 |
4 |
4 |
| Individual or group work |
1 |
8 |
8 |
| Preparation for Final exam |
1 |
32 |
32 |
| Course hours |
14 |
3 |
42 |
| Preparation for Midterm exam |
1 |
30 |
30 |
| Laboratory (including preparation) |
|
|
|
| Final exam |
1 |
2 |
2 |
| Homework |
|
|
|
| Quiz |
1 |
0.5 |
0.5 |
| Article Presentation |
1 |
0.2 |
0.2 |
| Total Work Load | 120.7 |
| Total Work Load / 30 (h) | 4.02 |
| ECTS Credit of the Course | 4 |
|
| Language of Instruction | Turkish |
|---|
| Work Placement(s) | No |
|---|
