| Course Type | Elective Course |
|---|
| Course Level | Master's Degree With Thesis |
|---|
| Year of Study | - |
|---|
| Semester | - |
|---|
| ECTS | 6 |
|---|
| Name of Lecturer(s) |
- |
| Learning Outcomes |
At the end of this course, the students;
- Knows the antibody concept, structure ve diversity,
- Knows genetic basis of antibody diversity,
- Knows monoclonal antibody production pipeline,
- Knows cell fusion technology, purification and characterization,
- Knows chimeric antibodies,
- Knows recombinant DNA practices in hybridoma technology,
- Knows cloning vectors, host cells and cloning strategies,
- Knows selection of clones and identification of recombinant clones,
- Knows diagnosis and targeting strategies using antibodies
|
| Mode of Delivery | Face to face |
|---|
| Prerequisites | No |
|---|
| Recommended Optional Programme Components | None |
|---|
| Course Description | In this course the antibody concept, structure ve diversity, genetic basis of antibody diversity, monoclonal antibody production; cell fusion technology, purification and characterization, chimeric antibodies, recombinant DNA practices in hybridoma technology; cloning vectors, host cells and cloning strategies, selection of clones and identification of recombinant clones, diagnosis and targeting strategies using antibodies will be explained. |
|---|
| Course Contents |
| WEEK |
TOPICS |
|
1st Week |
The antibody concept, structure ve diversity, |
|
2nd Week |
Genetic basis of antibody diversity, |
|
3rd Week |
Monoclonal antibody production, |
|
4th Week |
Cell fusion technology, purification and characterization, |
|
5th Week |
Chimeric antibodies, |
|
6th Week |
Recombinant DNA practices in hybridoma technology, |
|
7th Week |
Cloning vectors, host cells and cloning strategies, |
|
8th Week |
Selection of clones and identification of recombinant clones, |
|
9th Week |
Diagnosis and targeting strategies using antibodies |
|
10th Week |
|
|
| Recommended or Required Reading | 1. Introduction to Biotechnology (3rd Edition). William J. Thieman, Michael A. Palladino, Benjamin Cummings Press, 2012.
2. Molecular Biotechnology, Principles and Applications of Recombinant DNA. Bernard R. Glick, Jack J. Pasternak, ASM Press, Washington, D.C., 2009.
|
|---|
| Planned Learning Activities and Teaching Methods |
- Lecture
- Discussion
- Questions/Answers
- Presentation
|
|---|
| Assessment Methods and Criteria |
|
Quantity |
Percentage (%) |
| Mid-term |
1 |
50 |
| Total(%) |
|
50 |
| Contribution of In-term Studies to Overall Grade(%) |
|
50 |
| Contribution of Final Examination to Overall Grade(%) |
|
50 |
| Total(%) |
|
100 |
|
| ECTS Allocated Based on Student Workload |
| Activities |
Quantity |
Duration (Hour) |
Total Workload (Hour) |
| Midterm exam |
1 |
2 |
2 |
| Preparation for Quiz |
1 |
5 |
5 |
| Individual or group work |
14 |
2 |
28 |
| Preparation for Final exam |
1 |
20 |
20 |
| Course hours |
14 |
3 |
42 |
| Preparation for Midterm exam |
1 |
11 |
11 |
| Laboratory (including preparation) |
|
|
|
| Final exam |
1 |
2 |
2 |
| Homework |
2 |
35 |
70 |
| Total Work Load | 180 |
| Total Work Load / 30 (h) | 6 |
| ECTS Credit of the Course | 6 |
|
| Language of Instruction | Turkish |
|---|
| Work Placement(s) | No |
|---|
