HISTOCHEMICAL TECHNIQUES

HIS508

Course Code: HIS508
Semester: -
Theory+Practice: 2 + 1
ECTS: 5

Course Type

Elective Course

Course Level

Master's Degree With Thesis

Year of Study

Semester

ECTS

Name of Lecturer(s)

Learning Outcomes

At the end of this course, the students;

Explain the steps of the preparation of the samples be analyzed under a microscope
Describes basic methods of histochemistry,
Describes the methods of immunohistochemistry
Distinguish prepared samples with histochemical techniques under microscope
Explains the reasons for the usage cell culture and stem cell tecnology

Mode of Delivery

Face to face

Prerequisites

Recommended Optional Programme Components

None

Course Description

This course covers techniques for examining tissue under a microscope

Course Contents

WEEK	TOPICS
1^st Week	Introduction to histochemistry
2^nd Week	Fixation - solutions
3^rd Week	Formaldehyde containing fixatives
4^th Week	HgCL and alcohol contaaining fixatives
5^th Week	Maceration and decalcification techniques
6^th Week	Routine tissue processing for light microscopy
7^th Week	Routine and special tissue processing for electron mciroscopy
8^th Week	Tissue proccessing for scanning electron microscopy
9^th Week	Staining techniques for histological examination
10^th Week	Connective tissue stains
11^th Week	Nervous tissue examination techniques
12^th Week	Blood stains
13^th Week	Staining methods for cellular components
14^th Week	Enyme histochemistry

Recommended or Required Reading

Küehnel, Wolfgang. (2003). Color Atlas of Cytology, Histology, and Microscopic Anatomy. Stuttgart - New York: Thieme Verlack
Fawcwett, Don W. (1994). A Textbook of Histology. New York - London: Chapman and Hall. Twelfh Edition
Gartner, Leslie P. (1997). Color Textbook of Histology. Philadhelphia - London: Saunders Company
Ross, Michael H. (2003). Histology A Textbook and Atlas. Philadelphia: Williams and
Wilkins. Fourth Edition.
Junqueria, Luis C. (2003). Basic Histology Text and Atlas. Philadelphia: McGraw-Hill
Companies. Tenth Edition.
Alberts, B. (2002). Molecular Biology of The Cell. New York: Garland Science. Fourth
Edition.
Demir R. (2001). Histolojik Boyama Teknikleri. Palme Yayıncılık - Ankara. Birinci baskı.

Planned Learning Activities and Teaching Methods

Lecture
Discussion
Questions/Answers
Experiment

Assessment Methods and Criteria

	Quantity	Percentage (%)
Mid-term	1	30
Practice	1	20
Oral Exam	1	10
Total(%)		60
Contribution of In-term Studies to Overall Grade(%)		60
Contribution of Final Examination to Overall Grade(%)		40
Total(%)		100

ECTS Allocated Based on Student Workload

Activities	Quantity	Duration (Hour)	Total Workload (Hour)
Midterm exam	1	1	1
Preparation for Quiz
Individual or group work	14	3	42
Preparation for Final exam	10	3	30
Course hours	12	2	24
Preparation for Midterm exam	7	2	14
Laboratory (including preparation)	3	2	6
Final exam	1	1	1
Homework	1	3	3
Presentation (including preperation)	1	4	4
Weekly Articles and Resource Research	10	2	20
Total Work Load			145
Total Work Load / 30 (h)			4.83
ECTS Credit of the Course			5

Language of Instruction

Turkish

Work Placement(s)

Programme Qualifications / Learning Outcomes

	LO1	LO2	LO3	LO4	LO5
K1
K2
K3
K4
K5
K6
K7
K8
K9	X	X	X	X	X
K10
K11
K12
K13
K14
K15
K16	X	X	X	X	X
K17
K18
K19
K20
K21
K22
K23
K24
K25
K26	X	X	X	X	X
K27	X	X	X	X	X

	LO1	LO2	LO3	LO4	LO5
K1
K2
K3
K4
K5
K6
K7
K8
K9	X	X	X	X	X
K10
K11
K12
K13
K14
K15
K16	X	X	X	X	X
K17
K18
K19
K20
K21
K22
K23
K24
K25
K26	X	X	X	X	X
K27	X	X	X	X	X

	LO1	LO2	LO3	LO4	LO5
K1
K2
K3
K4
K5
K6
K7
K8
K9	X	X	X	X	X
K10
K11
K12
K13
K14
K15
K16	X	X	X	X	X
K17
K18
K19
K20
K21
K22
K23
K24
K25
K26	X	X	X	X	X
K27	X	X	X	X	X

	LO1	LO2	LO3	LO4	LO5
K1
K2
K3
K4
K5
K6
K7
K8
K9	X	X	X	X	X
K10
K11
K12
K13
K14
K15
K16	X	X	X	X	X
K17
K18
K19
K20
K21
K22
K23
K24
K25
K26	X	X	X	X	X
K27	X	X	X	X	X