SPECIAL HISTOCHEMISTRY TECHNIQUES

PLT217

Course Code: PLT217
Semester: Third Term (Fall)
Theory+Practice: 2 + 2
ECTS: 4

Course Type

Compulsory Course

Course Level

Associate Degree

Year of Study

Semester

Third Term (Fall)

ECTS

Name of Lecturer(s)

Instructor Nazlı Gök

Learning Outcomes

At the end of this course, the students;

Explains the basic concepts of histochemistry, the purposes of special staining techniques, and their areas of application.
Classifies appropriate histochemical staining methods for carbohydrate, connective tissue, lipid, nerve, microorganism, and pigment structures, and explains their technical details.
Identifies possible technical problems in histochemical staining processes, explains their causes, and develops appropriate solution proposals.

Mode of Delivery

Face to face

Prerequisites

Recommended Optional Programme Components

None

Course Description

Course Scope and Purpose: This course aims to enable students to learn the basic principles of histochemical staining methods other than routine histochemical staining techniques used in Pathology Laboratory and to apply them on various biological samples. The course aims to enable students to learn the chemical properties specific to the specific cellular structure or tissue to be examined and to choose the appropriate histochemical technique. In this context, it is aimed for students to analyze the tissues or cellular structures stained with the special histochemical techniques they learn in the course in terms of technical accuracy and to propose appropriate solutions when necessary.

Course Contents

WEEK	TOPICS
1^st Week	Introduction to the Course Syllabus and Structure
2^nd Week	General Principles of Histochemistry
3^rd Week	Preparation of Tissues for Histochemical Staining
4^th Week	Carbohydrate Stains I
5^th Week	Carbohydrate Stains II
6^th Week	Connective Tissue Stains I
7^th Week	Connective Tissue Stains II
8^th Week	Midterm Exam
9^th Week	Lipid Stains
10^th Week	Nerve Stains
11^th Week	Microorganism Stains
12^th Week	Pigment Stains
13^th Week	Considerations for Selecting Stains to Be Applied to Tissues
14^th Week	Problems Encountered in Histotechnology and Their Solutions
15^th Week
16^th Week

Recommended or Required Reading

Histotechnology. Carson FL, Hladik C, ASCP, 2009.
Teory and Practice of Histological Techniques. J.D Bancroft, M. Gamble. Churchill Livingsstone, 2008

Planned Learning Activities and Teaching Methods

Lecture
Questions/Answers
Discussion
Experiment
Practice
Presentation
Problem Solving

Assessment Methods and Criteria

	Quantity	Percentage (%)
Mid-term	1	30
Assignment	1	10
Practice	1	20
Total(%)		60
Contribution of In-term Studies to Overall Grade(%)		60
Contribution of Final Examination to Overall Grade(%)		40
Total(%)		100

ECTS Allocated Based on Student Workload

Activities	Quantity	Duration (Hour)	Total Workload (Hour)
Midterm exam	1	1	1
Preparation for Quiz	0	0	0
Individual or group work	8	3	24
Preparation for Final exam	1	20	20
Course hours	14	2	28
Preparation for Midterm exam	5	3	15
Laboratory (including preparation)	14	2	28
Final exam	1	1	1
Homework	1	5	5
Total Work Load			122
Total Work Load / 30 (h)			4.06
ECTS Credit of the Course			4

Language of Instruction

Turkish

Work Placement(s)

Programme Qualifications / Learning Outcomes

	LO1	LO2	LO3
K1	X	X
K2
K3
K4
K5			X
K6
K7			X
K8
K9
K10
K11	X	X
K12
K13		X
K14
K15