| Course Type | Elective Course |
|---|
| Course Level | Associate Degree |
|---|
| Year of Study | - |
|---|
| Semester | - |
|---|
| ECTS | 3 |
|---|
| Name of Lecturer(s) |
|
| Learning Outcomes |
At the end of this course, the students;
- Explain the fundamental concepts of molecular biology and the structure and transmission of genetic information.
- Distinguish the basic molecular diagnostic techniques used in molecular pathology, including their principles and areas of application.
- Interpret the role and clinical significance of PCR-based, hybridization-based, and sequencing technologies in molecular pathology practice.
|
| Mode of Delivery | Face to face |
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| Prerequisites | No |
|---|
| Recommended Optional Programme Components | None |
|---|
| Course Description | Scope and Purpose of the Course: The Molecular Pathology Techniques course aims to teach basic molecular biology processes and techniques used in molecular pathology that will help students understand pathological processes at the cellular and genetic level. This course teaches the technical details, application and place of technologies such as DNA and RNA isolation and analysis techniques, PCR, qPCR, FISH and DNA sequencing, which are basic molecular biology methods. This course aims to provide students with the ability to know the details of molecular biology techniques, compare them and apply them to specific situations. |
|---|
| Course Contents |
| WEEK |
TOPICS |
|
1st Week |
ntroduction of the lesson plan, Cell and Genome |
|
2nd Week |
Basic Genetic Mechanisms-1 |
|
3rd Week |
Basic Genetic Mechanisms-2 |
|
4th Week |
DNA Isolation Techniques |
|
5th Week |
DNA Analysis |
|
6th Week |
RNA Isolation and Analysis |
|
7th Week |
Midterm Exam |
|
8th Week |
Electrophoretic Techniques |
|
9th Week |
Polymerase Chain Reaction ? PCR |
|
10th Week |
Real Time PCR (qPCR) |
|
11th Week |
Fluorescence In situ Hybridization (FISH) |
|
12th Week |
DNA Sequencing Techniques (Sanger and NGS) |
|
13th Week |
Molecular Pathology Laboratory and Clinical Applications |
|
14th Week |
General Review |
|
15th Week |
|
|
| Recommended or Required Reading | Coleman, W. B., & Tsongalis, G. J. (Eds.). (2009). Molecular pathology: the molecular basis of human disease. academic Press.
Davis, L. (2012). Basic methods in molecular biology. Elsevier.
|
|---|
| Planned Learning Activities and Teaching Methods |
- Lecture
- Questions/Answers
- Presentation
- Experiment
- Practice
|
|---|
| Assessment Methods and Criteria |
|
Quantity |
Percentage (%) |
| Mid-term |
1 |
30 |
| Assignment |
1 |
20 |
| Total(%) |
|
50 |
| Contribution of In-term Studies to Overall Grade(%) |
|
50 |
| Contribution of Final Examination to Overall Grade(%) |
|
50 |
| Total(%) |
|
100 |
|
| ECTS Allocated Based on Student Workload |
| Activities |
Quantity |
Duration (Hour) |
Total Workload (Hour) |
| Midterm exam |
1 |
1 |
1 |
| Preparation for Quiz |
|
|
|
| Individual or group work |
|
|
|
| Preparation for Final exam |
1 |
25 |
25 |
| Course hours |
14 |
3 |
42 |
| Preparation for Midterm exam |
1 |
15 |
15 |
| Laboratory (including preparation) |
|
|
|
| Final exam |
1 |
1 |
1 |
| Homework |
1 |
10 |
10 |
| Total Work Load | 94 |
| Total Work Load / 30 (h) | 3.13 |
| ECTS Credit of the Course | 3 |
|
| Language of Instruction | Turkish |
|---|
| Work Placement(s) | No |
|---|
