Information Package

Program of Pharmacy

ECZ253

ANALYTICAL CHEMISTRY LABORATORY II

Course Code
ECZ253
Semester
Third Term (Fall)
Theory+Practice
0 + 3
ECTS
3
Course TypeCompulsory Course
Course LevelBachelor's Degree
Year of Study2
SemesterThird Term (Fall)
ECTS3
Name of Lecturer(s)
Learning Outcomes

At the end of this course, the students;

  1. Knows the definition of various instrument analysis methods. Makes qualitative and quantitative analysis of organic and inorganic materials with various chromatographic, spectroscopic and electrochemical methods. Gain knowledge and practical skills using various instrumental analysis methods. Learns how to make active substance (s) analysis from pharmaceutical preparations and drug and metabolite (s) analysis from biological materials (blood, urine, tissue etc.).
  2. Knows general information about thin layer and column chromatography and makes qualitative analysis of drugs with this method. Analyzes some transition metal ion mixtures by ion exchange chromatography.
  3. Knows the general information about ultraviolet - visible region (UV - GB) and derivative spectrophotometry methods and makes quantitative analysis of drugs and two-component mixtures with these methods.
  4. Determination of complex formulas and stability constants by spectrophotometric method (JOB Method)
  5. Knows general information about potentiometric titration and calculates the acidic equilibrium constants of a polyprotic acid with this method.
  6. Knows general information about conductivity titration and makes quantitative analysis of strong and weak acids with this method.
  7. Knows the validation parameters for method validity.
  8. Makes application studies on the parameters related to validation through some articles and experiment.
  9. After graduation, he can use the information he learned in this course in his work fields.
Mode of DeliveryFace to face
PrerequisitesNo
Recommended Optional Programme ComponentsNone
Course DescriptionQualitative and quantitative analysis of organic and inorganic substances by various chromatographic, spectroscopic and electrochemical methods.
Course Contents
WEEK TOPICS
1st Week Demonstration of spectroscopic, chromatographic and electrochemical methods
2nd Week Quiz and Qualitative analysis of drugs by thin layer chromatography at Week 2
3rd Week Quiz and Separation of indicator mixtures by column chromatography
4th Week Quiz and Analysis of some transition metal ion mixtures by Ion Exchange chromatography
5th Week Quiz and Quantitative analysis of drugs by UV-GB spectroscopy method (Paracetamol Quantitative Determination)
6th Week Quiz and Spectrophotometric quantification of two-component mixtures
7th Week Midterm exam week
8th Week Quiz and Analysis of two-component mixtures by derivative UV spectrophotometry
9th Week Quiz and Determination of complex formulas and stability constants by spectrophotometric method (JOB Method)
10th Week Quiz and Analysis of strong and weak acids by conductivity titration
11th Week Quiz and Determination of acidity equilibrium constants of a polyprotic acid by potentiometric titration
12th Week Validation demonstration
13th Week Practical studies on parameters related to validation through some articles and experiments
14th Week Quiz and Discussion of validation assignments
Recommended or Required Reading1. D.A. Skoog, F.J. Holler, S.R. Crouch, Principles Instrumental Analysis, Sixth Edition, Brooks Cole, USA, 2016.
2. H.H. Willard, L.L. Merritt, J.A. Dean, F.A. Settle, Instrumental Methods of Analysis, Sixth Edition, Litton Educational, USA, 1981.
3. OTHER RELATED CURRENT GUIDES, ARTICLES AND BOOKS
Planned Learning Activities and Teaching Methods
  • Lecture
  • Discussion
  • Report Preparation and/or Presentation
  • Experiment
Assessment Methods and Criteria
Quantity Percentage (%)
Assignment 1 10
Quiz 10 10
Practice 10 30
Total(%) 50
Contribution of In-term Studies to Overall Grade(%) 50
Contribution of Final Examination to Overall Grade(%) 50
Total(%) 100
ECTS Allocated Based on Student Workload
Activities Quantity Duration (Hour) Total Workload (Hour)
Midterm exam 0 0 0
Preparation for Quiz 10 1 10
Individual or group work 14 2 28
Preparation for Final exam 1 8 8
Course hours 0 0 0
Preparation for Midterm exam 0 0 0
Laboratory (including preparation) 14 3 42
Final exam 1 2 2
Homework 1 4 4
Quiz 10 0.25 2.5
Total Work Load96.5
Total Work Load / 30 (h)3.21
ECTS Credit of the Course3
Language of InstructionTurkish
Work Placement(s)No
LO1 LO2 LO3 LO4 LO5 LO6 LO7 LO8 LO9
K1 X
K2 X X X X X X X X X
K3 X X X X X X X X X
K4 X X X X X X X X X
K5 X X X X X X X X X
K6
K7
K8
K9 X X X X X X X X X
K10
K11
K12
K13
K14
K15
K16 X X X X X X X X X