BASIC PRINCIPLES OF LABORATORY TECHNIQUES

MBIO533

Course Code: MBIO533
Semester: -
Theory+Practice: 0 + 3
ECTS: 8

Course Type

Elective Course

Course Level

Master's Degree Without Thesis

Year of Study

Semester

ECTS

Name of Lecturer(s)

Learning Outcomes

At the end of this course, the students;

Mode of Delivery

Face to face

Prerequisites

Yes(MBIO531)

Recommended Optional Programme Components

Course Description

This course aims the introducition of the comman devices and equipments in Medical Biology Research Laboratory, preparing buffers for experiments and analyzing quality and quantity of nucleic acids and proteins will be discussed.

Course Contents

WEEK	TOPICS
1^st Week	DNA, RNA and Protein Structure
2^nd Week	Isolation Methods (DNA, RNA, Protein)
3^rd Week	PCR and PCR Based Techniques
4^th Week	PCR and PCR Based Techniques
5^th Week	Electrophoresis Systems and Electrophoresis Based Techniques
6^th Week	Class discussion with the recent review articles
7^th Week	Cell Culture Systems
8^th Week	Cell Culture Systems
9^th Week	Protein Imaging Methods
10^th Week	Protein Imaging Methods
11^th Week	Spectrophotometric Methods
12^th Week	Class discussion with the recent review articles
13^th Week	Class discussion with the recent review articles
14^th Week	Class discussion with the recent review articles

Recommended or Required Reading

Planned Learning Activities and Teaching Methods

Lecture
Discussion
Questions/Answers

Assessment Methods and Criteria

	Quantity	Percentage (%)
Mid-term	1	40
Total(%)		40
Contribution of In-term Studies to Overall Grade(%)		40
Contribution of Final Examination to Overall Grade(%)		60
Total(%)		100

ECTS Allocated Based on Student Workload

Activities	Quantity	Duration (Hour)	Total Workload (Hour)
Midterm exam	1	2.5	2.5
Preparation for Quiz	0	0	0
Individual or group work	14	10	140
Preparation for Final exam	1	24	24
Course hours	14	0	0
Preparation for Midterm exam	1	20	20
Laboratory (including preparation)	14	3	42
Final exam	1	2.5	2.5
Homework	1	8	8
Total Work Load			239
Total Work Load / 30 (h)			7.96
ECTS Credit of the Course			8

Language of Instruction

Turkish

Work Placement(s)

Programme Qualifications / Learning Outcomes

	LO1
K1	X
K2	X
K3	X
K4	X
K5	X
K6	X
K7	X
K8	X
K9	X