knows and applies DNA and RNA isolation methods from tissues
knows and applies the nucleic acid scanning by electrophoresis
knows and applies the PCR based investigation methods
knows and applies the restriction enzime cutting techniques
knows and applies the DNA sequencing techniques
knows and applies the microarray tecniques
Mode of Delivery
Face to face
Prerequisites
No
Recommended Optional Programme Components
Course Description
In laboratory practices students are supposed to choose a research project topic according to their interests, write the project and complete experimental processes. Throughout the research, students are expected to apply basic molecular techniques such as total DNA and RNA isolation from cells and tissues, electrophoretic visualization of nucleic acids, polymerase chain reaction based research techniques (molecular cloning of genomic regions, reverse-transcriptase PCR, Real Time PCR), restriction enzyme digestion and its applications, DNA sequencing techniques and microarray technology.
Course Contents
In laboratory practices students are supposed to choose a research project topic according to their interests, write the project and complete experimental processes. Throughout the research, students are expected to apply basic molecular techniques such as total DNA and RNA isolation from cells and tissues, electrophoretic visualization of nucleic acids, polymerase chain reaction based research techniques (molecular cloning of genomic regions, reverse-transcriptase PCR, Real Time PCR), restriction enzyme digestion and its applications, DNA sequencing techniques and microarray technology.
Recommended or Required Reading
? Principles of Gene Manipulation and Genomics, 8th Edition. Sandy B. Primrose, Richard Twyman (2010).
? Human Molecular Genetics, 4th Edition. Strachan T., Read A. Garland Science (2010).
Planned Learning Activities and Teaching Methods
Case Study
Problem Solving
Experiment
Practice
Discussion
Project
Report Preparation
Presentation
Assessment Methods and Criteria
Quantity
Percentage (%)
Mid-term
1
30
Assignment
1
20
Total(%)
50
Contribution of In-term Studies to Overall Grade(%)
50
Contribution of Final Examination to Overall Grade(%)
