Know the isolation of DNA and total RNA from cells and tissues, and apply
Know electrophoretic display of nucleic acids and apply
know polimerase chain reaction based techniques (molecular cloning of genomic sites, reverse-transcription PCR, real-time PCR)and apply
know restriction enzyme digestion and apply
Know DNA sequencing techniques and microarray technology and apply them
Evaluate molecular genetic techniques and scientific data related to their analysis.
Mode of Delivery
Face to face
Prerequisites
No
Recommended Optional Programme Components
Course Description
This course will cover DNA and total RNA isolation from cells and tissues, electrophoresis of nucleic acids, polimerase chain reaction based techniques (molecular cloning of genomic sites, reverse-transcription PCR, real-time PCR), restriction enzyme digestion and its applications, DNA sequencing, microarray technology and their laboratory practices.
Course Contents
This course will cover DNA and total RNA isolation from cells and tissues, electrophoresis of nucleic acids, polimerase chain reaction based techniques (molecular cloning of genomic sites, reverse-transcription PCR, real-time PCR), restriction enzyme digestion and its applications, DNA sequencing, microarray technology and their laboratory practices.
Recommended or Required Reading
Principles of Gene Manipulation and Genomics (7th edition). Sandy B. Primrose, Richard Twyman, Wiley-Blackwell, 2006.
Planned Learning Activities and Teaching Methods
Lecture
Discussion
Questions/Answers
Problem Solving
Practice
Presentation
Case Study
Assessment Methods and Criteria
Quantity
Percentage (%)
Mid-term
1
30
Assignment
1
20
Total(%)
50
Contribution of In-term Studies to Overall Grade(%)
50
Contribution of Final Examination to Overall Grade(%)
