PROCARYOTIC AND EUKARYOTIC CULTURE TECHNIQUES

BME619

Course Code: BME619
Semester: -
Theory+Practice: 3 + 0
ECTS: 10

Course Type

Elective Course

Course Level

Doctorate Of Science

Year of Study

Semester

ECTS

Name of Lecturer(s)

Professor Özlem Darcansoy İşeri

Learning Outcomes

At the end of this course, the students;

To learn similarities and differences of prokaryotic and eukaryotic cells
To understand the fundamentals of basic aseptic techniques, sterilization and applications
To learn fundamentals of prokaryotic cell and plant tissue culture techniques and their biomedical applications
To learn 2-D and 3-D animal tissue culture techniques and their biomedical applications
To have knowledge on the cytotoxicity and genotoxicity studies using culture techniques
To discuss current applications on an individually assigned project basis

Mode of Delivery

Face to face

Prerequisites

Recommended Optional Programme Components

None

Course Description

Prokaryotic and Eukaryotic Cells; Basic Aseptic Techniques and Sterilization Methods; Cell Culture Types, Basic Concepts, Laboratory Design and Instrumentation; Prokaryotic Culture Media, Media, Culture Techniques Biomedical Applications of Prokaryotic Cultures; Plant Tissue Culture Media, Media, Culture Techniques Biomedical Applications of Plant Tissue Cultures; Animal Cell Cultures, Media and Equipment; Basic Techniques in Animal Cell Culture; Three Dimensional Cell and Tissue Cultures; Biomedical Applications of Animal Tissue Cultures Cytotoxicity and Genotoxicity Studies

Course Contents

WEEK	TOPICS
1^st Week	Prokaryotic and Eukaryotic Cells
2^nd Week	Basic Aseptic Techniques and Sterilization
3^rd Week	Introduction to and Concepts of Cell Culture, Laboratory Design and Instrumentation
4^th Week	Prokaryotic Cultures, Mediums and Culture Techniques
5^th Week	Biomedical Applications of Prokaryotic Cultures
6^th Week	Plant Tissue Cultures, Mediums and Culture Techniques
7^th Week	Biomedical Applications of Plant Tissue Cultures
8^th Week	Midterm Week
9^th Week	Animal Cell Cultures, Mediums and Equipment
10^th Week	Basic Techniques in Animal Cell Culture
11^th Week	3-D Cell and Tissue Culture
12^th Week	Biomedical Applications of Animal Cell Cultures
13^th Week	Cytotoxicity and Genotoxicity Studies
14^th Week	Presentations

Recommended or Required Reading

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. 6th edition. R. Ian Freshney.
Internet and library files.

Planned Learning Activities and Teaching Methods

Lecture
Discussion
Presentation

Assessment Methods and Criteria

	Quantity	Percentage (%)
Mid-term	1	35
Assignment	1	15
Total(%)		50
Contribution of In-term Studies to Overall Grade(%)		50
Contribution of Final Examination to Overall Grade(%)		50
Total(%)		100

ECTS Allocated Based on Student Workload

Activities	Quantity	Duration (Hour)	Total Workload (Hour)
Midterm exam	1	72	72
Preparation for Quiz	0	0	0
Individual or group work	2	3	6
Preparation for Final exam	1	24	24
Course hours	14	3	42
Preparation for Midterm exam	1	24	24
Laboratory (including preparation)	0	0	0
Final exam	1	96	96
Homework	1	24	24
Total Work Load			288
Total Work Load / 30 (h)			9.6
ECTS Credit of the Course			10

Language of Instruction

English

Work Placement(s)

Programme Qualifications / Learning Outcomes

	LO1	LO2	LO3	LO4	LO5	LO6
K1	X	X		X	X	X
K2
K3
K4	X	X	X	X	X	X
K5
K6						X
K7
K8						X